Technical Data

032453
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu Mo
Accession #
NP_033869.1; O55028
Gene ID
12041
Gene #
Bckdk
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Bckdk, ID (Bckdk, [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial, Branched-chain alpha-ketoacid dehydrogenase kinase)
Bckdk, [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial, Branched-chain alpha-ketoacid dehydrogenase kinase

Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex (By similarity).

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Mouse Bckdk antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-188 amino acids from the Central region of Mouse Bckdk.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human, mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Pagliarini, D.J., et al. Cell 134(1):112-123(2008)|Lee, J., et al. Mol. Cell Proteomics 6(4):669-676(2007)|Hutson, S.M. Biochem. J. 400 (1), E1-E3 (2006) :|Joshi, M.A., et al. Biochem. J. 400(1):153-162(2006)|Trinidad, J.C., et al. Mol. Cell Proteomics 5(5):914-922(2006)
USBio References
No references available
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