032467
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
PurifiedApplications
E FC IHC WBCrossreactivity
HuGene ID
597Gene #
BCL2A1Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-BCL2A1, ID (BCL2A1, BCL2L5, BFL1, GRS, HBPA1, Bcl-2-related protein A1, Bcl-2-like protein 5, Hemopoietic-specific early response protein, Protein BFL-1, Protein GRS)
BCL2A1, BCL2L5, BFL1, GRS, HBPA1, Bcl-2-related protein A1, Bcl-2-like protein 5, Hemopoietic-specific early response protein, Protein BFL-1, Protein GRS
Bcl-2 related Protein A1 is a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators that are involved in a wide variety of cellular activities such as embryonic development, homeostasis and tumorigenesis. The protein encoded by this gene is able to reduce the release of pro-apoptotic cytochrome c from mitochondria and block caspase activation. This gene is a direct transcription target of NF-kappa B in response to inflammatory mediators, and has been shown to be up-regulated by different extracellular signals, such as granulocyte-macrophage colony-stimulating factor (GM-CSF), CD40, phorbol ester and inflammatory cytokine TNF and IL-1, which suggests a cytoprotective function that is essential for lymphocyte activation as well as cell survival.
Applications
Suitable for use in Western Blot, Immunohistochemistry, Indirect Flow Cytometry and ELISA Other applications not tested.
Recommended Dilution
Western Blot: 1:50-100 Immunohistochemistry: 1:50-100 Indirect Flow Cytometry: 1:10-50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 63-92 from the Center region of human BCL2A1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Specificity
Recognizes human BCL2A1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Akatsuka, Y., et al., J. Exp. Med. 197(11):1489-1500 (2003). 2. Edelstein, L.C., et al., Mol. Cell. Biol. 23(8):2749-2761 (2003). 3. Werner, A.B., et al., J. Biol. Chem. 277(25):22781-22788 (2002). 4. Akari, H., et al., J. Exp. Med. 194(9):1299-1311 (2001). 5. Harrington, J.J., et al., Nat. Biotechnol. 19(5):440-445 (2001).USBio References
No references available