Technical Data

032475
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_055554.1; NP_001070908.1; NP_001070909.1
Gene ID
9774
Gene #
BCLAF1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-BCLAF1, CT (BCLAF1, BTF, KIAA0164, Bcl-2-associated transcription factor 1)
BCLAF1, BTF, KIAA0164, Bcl-2-associated transcription factor 1

This gene encodes a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins. The protein localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.

Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
BCLAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 658-688 amino acids from the C-terminal region of human BCLAF1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Sarras, H., et al. ScientificWorldJournal 10, 1450-1461 (2010) :|Renert, A.F., et al. J. Proteome Res. 8(10):4810-4822(2009)|Liu, H., et al. Mol. Cell. Biol. 27(24):8480-8491(2007)|Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)|Olsen, J.V., et al. Cell 127(3):635-648(2006)
USBio References
No references available
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