Technical Data

032498
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_116124.2
Gene ID
144453
Gene #
BEST3
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-BEST3, NT (BEST3, VMD2L3, Bestrophin-3, Vitelliform macular dystrophy 2-like protein 3)
BEST3, VMD2L3, Bestrophin-3, Vitelliform macular dystrophy 2-like protein 3

BEST3 belongs to the bestrophin family of anion channels, which includes BEST1 (MIM 607854), the gene mutant in vitelliform macular dystrophy (VMD; MIM 153700), and 2 other BEST1-like genes, BEST2 (MIM 607335) and BEST4 (MIM 607336). Bestrophins are transmembrane (TM) proteins that share a homology region containing a high content of aromatic residues, including an invariant arg-phe-pro (RFP) motif. The bestrophin genes share a conserved gene structure, with almost identical sizes of the 8 RFP-TM domain-encoding exons and highly conserved exon-intron boundaries. Each of the 4 bestrophin genes has a unique 3-prime end of variable length (Stohr et al., 2002 [PubMed 12032738]; Tsunenari et al., 2003 [PubMed 12907679]).

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
BEST3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-69 amino acids from the N-terminal region of human BEST3.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Marsey, L.L., et al. J. Physiol. (Lond.) 587 (PT 10), 2211-2224 (2009) :|Qu, Z.Q., et al. J. Biol. Chem. 282(24):17460-17467(2007)|Tsunenari, T., et al. J. Biol. Chem. 278(42):41114-41125(2003)|Stohr, H., et al. Eur. J. Hum. Genet. 10(4):281-284(2002)|Marquardt, A., et al. Hum. Mol. Genet. 7(9):1517-1525(1998)
USBio References
No references available
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