Rabbit Anti-BHLHB5, ID (BHLHE22, BHLHB5, TNRC20, Class E basic helix-loop-helix protein 22, Class B basic helix-loop-helix protein 5, Trinucleotide repeat-containing gene 20 protein)
BHLHE22, BHLHB5, TNRC20, Class E basic helix-loop-helix protein 22, Class B basic helix-loop-helix protein 5, Trinucleotide repeat-containing gene 20 protein
BHLHB5 inhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. BHLHB5 despite the presence of an intact basic domain, does not bind to DNA.
Applications
Suitable for use in Western Blot, Immunohistochemistry, Indirect Flow Cytometry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Indirect Flow Cytometry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
BHLHB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243~272 amino acids from the Central region of human BHLHB5.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
