Rabbit Anti-BPGM, CT (BPGM, Bisphosphoglycerate mutase, 2,3-bisphosphoglycerate mutase, erythrocyte, 2,3-bisphosphoglycerate synthase, 2,3-diphosphoglycerate mutase, BPG-dependent PGAM) (Biotin)
BPGM, Bisphosphoglycerate mutase, 2,3-bisphosphoglycerate mutase, erythrocyte, 2,3-bisphosphoglycerate synthase, 2,3-diphosphoglycerate mutase, BPG-dependent PGAM
2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also has phosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen. Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 230-259 in the C-terminal region of human BPGM. Species Sequence Homology: monkey
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human BPGM.
