Technical Data

033180
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E FC IHC WB
Crossreactivity
Hu
Gene ID
825
Gene #
CAPN3
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-CAPN3, CT (CAPN3, CANPL3, CANP3, NCL1, Calpain-3, Calcium-activated neutral proteinase 3, Calpain L3, Calpain p94, Muscle-specific calcium-activated neutral protease 3, New calpain 1)
CAPN3, CANPL3, CANP3, NCL1, Calpain-3, Calcium-activated neutral proteinase 3, Calpain L3, Calpain p94, Muscle-specific calcium-activated neutral protease 3, New calpain 1

Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A.

Applications
Suitable for use in Western Blot, Immunohistochemistry, Indirect Flow Cytometry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Formalin fixed, paraffin embedded tissues Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 603-632 in the C-terminal region of human CAPN3. Species Sequence Homology: monkey
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Specificity
Recognizes human CAPN3

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Charlton, R., et al. Neuromuscul. Disord. 19(7):449-457(2009). 2. Moretti, D., et al. Carcinogenesis 30(6):960-967(2009). 3. Enjuanes, A., et al. Cancer Res. 68(24):10178-10186(2008). 4. Beckmann, J.S., et al. Neuromuscul. Disord. 18(12):913-921(2008). 5. Shirafuji, T., et al. Rinsho Shinkeigaku 48(9):651-655(2008). 6. Diaz, B.G., et al. J. Biol. Chem. 279(26):27656-27666(2004)
USBio References
No references available
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