Rabbit Anti-Casc5, CT (Casc5, Knl1, Protein CASC5, Cancer susceptibility candidate gene 5 protein homolog, Kinetochore-null protein 1)
Casc5, Knl1, Protein CASC5, Cancer susceptibility candidate gene 5 protein homolog, Kinetochore-null protein 1
Performs two crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Directly links spindle checkpoint proteins BUB1 and BUB1B to kinetochores. Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore (By similarity).
Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Mouse Casc5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1556-1583 amino acids from the C-terminal region of mouse Casc5.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
