Technical Data

033253
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Gene ID
8535
Gene #
CBX4
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-CBX4, CT (CBX4, E3 SUMO-protein ligase CBX4, Chromobox protein homolog 4, Polycomb 2 homolog)
CBX4, E3 SUMO-protein ligase CBX4, Chromobox protein homolog 4, Polycomb 2 homolog

Chromobox homolog 4 (CBX4 or Pc2) is a member of Drosophila Polycomb group gene family. The polycomb group (PcG) genes are essential for maintenance of appropriate expression patterns of developmental master regulators and thus are essential for proper development. Changes in expression pf PcG proteins have been associated with cancer. CBX4 is a part of the cellular memory system that is responsible for the inheritance of gene activity by progeny cells. CBX4 is involved in maintaining the transcriptionally repressive state of genes. It modifies chromatin, rendering it heritably changed in its expressibility. Structurally, CBX4 contains 1 chromo domain. It was reported that Pc2 is involved in sumoylation process by recruiting SUMO E1 and E2, and Pc2 is actually SUMO E3 during sumoylation events. CBX4 expression is ubiquitous.

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
CBX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 415~444 amino acids from the C-terminal region of human CBX4.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Kagey, M.H., et al., Cell 113(1):127-137 (2003).|Satijn, D.P., et al., Mol. Cell. Biol. 17(10):6076-6086 (1997).
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net