033254
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
HuAccession #
O00257; NP_003646.2Gene ID
8535Gene #
CBX4Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-CBX4, NT (CBX4, E3 SUMO-protein ligase CBX4, Chromobox protein homolog 4, Polycomb 2 homolog)
CBX4, E3 SUMO-protein ligase CBX4, Chromobox protein homolog 4, Polycomb 2 homolog
E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
CBX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CBX4.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Seong, I.S., et al. Hum. Mol. Genet. 19(4):573-583(2010)|Zhou, W., et al. BMB Rep 42(3):154-159(2009)|Liang, G., et al. Hum. Mol. Genet. 17(8):1109-1119(2008)|Kim, S.H., et al. Int. J. Biochem. Cell Biol. 40(11):2462-2471(2008)|Kabil, O., et al. Biochemistry 45(45):13528-13536(2006)USBio References
No references available