Technical Data

033372
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_114172.1
Gene ID
891
Gene #
CCNB1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-CCNB1, CT (CCNB1, CCNB, G2/mitotic-specific cyclin-B1)
CCNB1, CCNB, G2/mitotic-specific cyclin-B1

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq].

Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-100 Immunohistochemistry: 1:50-100
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
CCNB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 388-418 amino acids from the C-terminal region of human CCNB1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Kreis, N.N., et al. Oncogene 29(41):5591-5603(2010)|van Zon, W., et al. J. Cell Biol. 190(4):587-602(2010)|Harley, M.E., et al. EMBO J. 29(14):2407-2420(2010)|Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :|Nantajit, D., et al. PLoS ONE 5 (8), E12341 (2010) :
USBio References
No references available
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