Technical Data

033433-FITC
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Conjugate
FITC
Isotype
IgG
Grade
Affinity Purified
Applications
FLISA WB
Crossreactivity
Hu
Accession #
NP_005192.1
Gene ID
1237
Gene #
CCR8
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Rabbit Anti-CCR8, CT (CCR8, CKRL1, CMKBR8, CMKBRL2, C-C chemokine receptor type 8, CC chemokine receptor CHEMR1, Chemokine receptor-like 1, GPR-CY6, TER1, CDw198) (FITC)
CCR8, CKRL1, CMKBR8, CMKBRL2, C-C chemokine receptor type 8, CC chemokine receptor CHEMR1, Chemokine receptor-like 1, GPR-CY6, TER1, CDw198

CCR8 encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are important for the migration of various cell types into the inflammatory sites. This receptor protein preferentially expresses in the thymus. I-309, thymus activation-regulated cytokine (TARC) and macrophage inflammatory protein-1 beta (MIP-1 beta) have been identified as ligands of this receptor. Studies of this receptor and its ligands suggested its role in regulation of monocyte chemotaxis and thymic cell apoptosis. More specifically, this receptor may contribute to the proper positioning of activated T cells within the antigenic challenge sites and specialized areas of lymphoid tissues. CCR8 is located at the chemokine receptor gene cluster region.

Applications
Suitable for use in Western Blot and FLISA. Other potential application, though not tested is Direct Flow Cytometry. Other applications have not been tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 305-335 in the C-terminal region of human CCR8.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human CCR8.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Mutalithas, K., et al. Clin. Exp. Allergy 40(8):1175-1185(2010) Han, S., et al. Hum. Immunol. 71(7):727-730(2010) Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1356-1361(2010) Davila, S., et al. Genes Immun. 11(3):232-238(2010) Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 18(5):1651-1658(2009)
USBio References
No references available
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