Technical Data

033449-PE
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Conjugate
PE
Isotype
IgG
Grade
Affinity Purified
Applications
FLISA IHC WB
Crossreactivity
Hu
Accession #
NP_000623
Gene ID
3684
Gene #
ITGAM
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
Rabbit Anti-CD11b, NT (ITGAM, CD11B, CR3A, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b) (PE)
ITGAM, CD11B, CR3A, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b

CD11b is the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles.

Applications
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry (Not Tested) and FLISA. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 253-282 in the N-terminal region of human CD11b, UniProt Accession # P11215.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human CD11b.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Gjelstrup, L.C., et al. J. Immunol. 185(7):4154-4168 (2010) 2. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253 (2010) 3. Warchol, T., et al. DNA Cell Biol. (2010). 4. Fan, Y., et al. J Eur Acad Dermatol Venereol (2010). 5. Pliyev, B.K., et al. Biochem. Biophys. Res. Commun. 397(2):277-282 (2010)
USBio References
No references available
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