Technical Data

033638
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Gene ID
983
Gene #
CDK1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-CDK1 (T14) (CDK1, CDC2, CDC28A, CDKN1, P34CDC2, Cyclin-dependent kinase 1, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase)
CDK1, CDC2, CDC28A, CDKN1, P34CDC2, Cyclin-dependent kinase 1, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase

CDK1 is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control.

Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-100 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
CDK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T14 of human CDK1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by peptide immunoaffinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Kramer, A., et al., Nat. Cell Biol. 6(9):884-891 (2004).|Dai, X., et al., J. Invest. Dermatol. 122(6):1356-1364 (2004).|Litvak, V., et al., Mol. Cell 14(3):319-330 (2004).|Shapira, M., et al., Cancer 100(8):1615-1621 (2004).|Chow, J.P., et al., J. Biol. Chem. 278(42):40815-40828 (2003).
USBio References
No references available
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