Technical Data

033734
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
DB E IF
Crossreactivity
Hu
Gene ID
1045
Gene #
CDX2
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-CDX2, phosphorylated (S283) (CDX2, CDX3, Homeobox protein CDX-2, CDX-3, Caudal-type homeobox protein 2)
CDX2, CDX3, Homeobox protein CDX-2, CDX-3, Caudal-type homeobox protein 2

The level and beta-cell specificity of insulin gene expression are regulated by a set of nuclear proteins that bind to specific sequences within the promoter of the insulin gene (INS; MIM 176730) and interact with RNA polymerase to activate or repress transcription. The proteins LMX1 (MIM 600298) and CDX3 are homeodomain proteins that bind an A/T-rich sequence in the insulin promoter and stimulate its transcription.

Applications
Suitable for use in Immunofluorescence, Dot Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Dot Blot 1:500 Immunofluorescence: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Phospho-CDX2-S283 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 268~297 amino acids surrounding S283 of human CDX2.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Benoit, Y.D., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 298 (4), G504-G517 (2010) |Xie, Y., et al. Int. J. Oncol. 36(2):509-516(2010)|Park do, Y., et al. Mod. Pathol. 23(1):54-61(2010)|Lora, V., et al. Anticancer Res. 29(12):5033-5037(2009)|Porjazova, E., et al. Akush Ginekol (Sofiia) 48(4):32-34(2009)
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net