Technical Data

033811
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E FC IHC WB
Crossreactivity
Hu Mo
Gene ID
1072
Gene #
CFL1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-CFL1, ID (CFL1, CFL, Cofilin-1, 18kD phosphoprotein, Cofilin, non-muscle isoform)
CFL1, CFL, Cofilin-1, 18kD phosphoprotein, Cofilin, non-muscle isoform

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner.

Applications
Suitable for use in Western Blot, Indirect Flow Cytometry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Indirect Flow Cytometry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
CFL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77~106 amino acids from the Center region of human CFL1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human, mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Fazal,F., et.al., J. Biol. Chem. 284 (31), 21047-21056 (2009)
USBio References
No references available
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