Technical Data

033869
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_065135.2
Gene ID
57053
Gene #
CHRNA10
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-CHRNA10, ID (CHRNA10, NACHRA10, Neuronal acetylcholine receptor subunit alpha-10, Nicotinic acetylcholine receptor subunit alpha-10)
CHRNA10, NACHRA10, Neuronal acetylcholine receptor subunit alpha-10, Nicotinic acetylcholine receptor subunit alpha-10

CHRNA10 is an ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding may induce an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this may lead to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma.

Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
CHRNA10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-214 amino acids from the Central region of human CHRNA10.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Saccone, N.L., et al. Genes Brain Behav. 9(7):741-750(2010)|Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :|Rigbi, A., et al. Pharmacogenomics J. (2010) In press :|Need, A.C., et al. Eur. J. Hum. Genet. 17(7):946-957(2009)|Saccone, N.L., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 150B (4), 453-466 (2009) :
USBio References
No references available
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