Technical Data

034079
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_004636.1
Gene ID
8161
Gene #
COIL
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-COIL, ID (COIL, CLN80, Coilin, p80)
COIL, CLN80, Coilin, p80

The protein encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14.

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
COIL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-186 amino acids from the Central region of human COIL.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Shanbhag, R., et al. FEBS Lett. 584(20):4351-4356(2010)|Tapia, O., et al. Chromosoma 119(5):527-540(2010)|Toyota, C.G., et al. Chromosoma 119(2):205-215(2010)|Zhao, J., et al. BMC Med. Genet. 11, 96 (2010) :|Hearst, S.M., et al. J. Cell. Sci. 122 (PT 11), 1872-1881 (2009) :
USBio References
No references available
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