Technical Data

034098
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_001841.2; NP_065084.2
Gene ID
1295
Gene #
COL8A1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-COL8A1, NT (COL8A1, C3orf7, Collagen alpha-1(VIII) chain, Endothelial collagen, Vastatin)
COL8A1, C3orf7, Collagen alpha-1(VIII) chain, Endothelial collagen, Vastatin

This gene encodes one of the two alpha chains of type VIII collagen. The gene product is a short chain collagen and a major component of the basement membrane of the corneal endothelium. The type VIII collagen fibril can be either a homo- or a heterotrimer. Alternatively spliced transcript variants encoding the same isoform have been observed.

Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 62-89 in the N-terminal region of human COL8A1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human COL8A1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) 2. Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) 3. Neale, B.M., et al. Proc. Natl. Acad. Sci. U.S.A. 107(16):7395-7400(2010) 4. Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009) 5. Marroni, F., et al. Circ Cardiovasc Genet 2(4):322-328(2009).
USBio References
No references available
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