034112
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E FC WBCrossreactivity
HuAccession #
NP_443121.1; NP_001017534.1;Gene ID
114769Gene #
CARD16Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-COP1, NT (CARD16, COP, COP1, Caspase recruitment domain-containing protein 16, CARD only domain-containing protein 1, Caspase-1 inhibitor COP, Pseudo interleukin-1 beta converting enzyme)
CARD16, COP, COP1, Caspase recruitment domain-containing protein 16, CARD only domain-containing protein 1, Caspase-1 inhibitor COP, Pseudo interleukin-1 beta converting enzyme
Caspase inhibitor. Acts as a regulator of procaspase-1/CASP1 activation implicated in the regulation of the proteolytic maturation of pro-interleukin-1 beta (IL1B) and its release during inflammation. Inhibits the release of IL1B in response to LPS in monocytes. Also induces NF-kappa-B activation during the pro-inflammatory cytokine response. Also able to inhibit CASP1-mediated neuronal cell death, TNF-alpha, hypoxia-, UV-, and staurosporine-mediated cell death but not ER stress-mediated cell death. Acts by preventing activation of caspases CASP1 and CASP4, possibly by preventing the interaction between CASP1 and RIPK2.
Applications
Suitable for use in Western Blot, Indirect Flow Cytometry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Indirect Flow Cytometry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
COP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44-73 amino acids from the N-terminal region of human COP1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Bailey, S.D., et al. Diabetes Care (2010) In press :|Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)|Wang, X., et al. Biochim. Biophys. Acta 1762(8):742-754(2006)|Wang, X., et al. J. Neurosci. 25(50):11645-11654(2005)|Lamkanfi, M., et al. J. Biol. Chem. 279(50):51729-51738(2004)USBio References
No references available