034115
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E IF IHC WBCrossreactivity
HuAccession #
NP_009194.2; O14579Gene ID
11316Gene #
COPEShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-COPE, CT (COPE, Coatomer subunit epsilon, Epsilon-coat protein)
COPE, Coatomer subunit epsilon, Epsilon-coat protein
The product of this gene is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.
Applications
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50 Immunofluorescence: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
COPE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 280-308 amino acids from the C-terminal region of human COPE.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Davila, S., et al. Genes Immun. 11(3):232-238(2010)|Morikawa, R.K., et al. J. Biol. Chem. 284(39):26620-26630(2009)|Maruyama, S., et al. Mol. Cell. Biochem. 307 (1-2), 73-82 (2008) :|Goryachev, A.B., et al. PLoS Comput. Biol. 2 (12), E172 (2006) :|Lippincott-Schwartz, J., et al. Trends Cell Biol. 16 (10), E1-E4 (2006) :USBio References
No references available