034116
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
HuAccession #
NP_057212.1Gene ID
22820Gene #
COPGShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-COPG, CT (COPG1, COPG, Coatomer subunit gamma-1, Gamma-1-coat protein)
COPG1, COPG, Coatomer subunit gamma-1, Gamma-1-coat protein
The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).
Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
COPG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 721-750 amino acids from the C-terminal region of human COPG.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Pinheiro, A.P., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010) :|Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :|Lippincott-Schwartz, J., et al. Trends Cell Biol. 16 (10), E1-E4 (2006) :|Watson, P.J., et al. Traffic 5(2):79-88(2004)|Rohde, H.M., et al. J. Biol. Chem. 278(52):52689-52699(2003)USBio References
No references available