Rabbit Anti-CPT1C, CT (CPT1C, CATL1, Carnitine O-palmitoyltransferase 1, brain isoform, CPT IC, Carnitine O-palmitoyltransferase I, brain isoform, Carnitine palmitoyltransferase 1C)
CPT1C, CATL1, Carnitine O-palmitoyltransferase 1, brain isoform, CPT IC, Carnitine O-palmitoyltransferase I, brain isoform, Carnitine palmitoyltransferase 1C
The Cpt1 family of proteins are outer mitochondrial membrane proteins that regulate the entry into, and oxidation of fatty acids by, mitochondria. Malonyl-CoA, an intermediate in fatty acid synthesis, has been implicated as a regulatory component of the energy sensing system that feeds into hypothalmic neurons to impart energy homeostasis. Malonyl-CoA levels in the hypothalamus are dynamically regulated by fasting and feeding, altering subsequent feeding behaviour. Cpt1c, the brain-specific carnitine O-palmitoyltransferase 1, is thought to relay information about malonyl-CoA levels in hypothalamic neurons that express orexigenic and anorexigenic neuropeptides that regulate food intake and peripheral energy expenditure. Unlike other Cpt1 proteins, Cpt1c binds Malonyl-CoA but does not catalyse the transfer of the malonyl group from CoA to carnitine.
Applications
Suitable for use in Western Blot, Indirect Flow Cytometry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Indirect Flow Cytometry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
CPT1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 759-790 amino acids from the C-terminal region of human CPT1C.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
