Technical Data

034242
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E FC IHC WB
Crossreactivity
Hu Mo
Gene ID
54677
Gene #
CROT
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-CROT, NT (CROT, COT, Peroxisomal carnitine O-octanoyltransferase)
CROT, COT, Peroxisomal carnitine O-octanoyltransferase

CROT encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described.

Applications
Suitable for use in Western Blot, Immunohistochemistry, Indirect Flow Cytometry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-100 Immunohistochemistry: 1:50-100 Indirect Flow Cytometry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
CROT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57~86 amino acids from the N-terminal region of human CROT.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Specificity
Recognizes human CROT. Species Crossreactivity: mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Li, Y.L., et al. FEBS J. 276(1):303-314(2009)|Morillas, M., et al. J. Biol. Chem. 277(13):11473-11480(2002)|van der Leij, F.R., et al. Mol. Genet. Metab. 71 (1-2), 139-153 (2000) :|Ferdinandusse, S., et al. Biochem. Biophys. Res. Commun. 263(1):213-218(1999)
USBio References
No references available
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