Technical Data

034411
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E FC WB
Crossreactivity
Hu Mo
Accession #
NP_001907.2
Gene ID
1537
Gene #
CYC1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-CYC1, CT (CYC1, Cytochrome c1, heme protein, mitochondrial, Complex III subunit 4, Complex III subunit IV, Cytochrome b-c1 complex subunit 4, Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit)
CYC1, Cytochrome c1, heme protein, mitochondrial, Complex III subunit 4, Complex III subunit IV, Cytochrome b-c1 complex subunit 4, Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit

This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain.

Applications
Suitable for use in Western Blot, Indirect Flow Cytometry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Indirect Flow Cytometry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
CYC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-299 amino acids from the C-terminal region of human CYC1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human, mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :|Cummings, C., et al. Apoptosis 11(7):1121-1129(2006)|Wen, J.J., et al. Free Radic. Biol. Med. 37(12):2072-2081(2004)|An, J., et al. J. Biol. Chem. 279(18):19133-19140(2004)|Yuan, J., et al. Biochim. Biophys. Acta 1641(1):35-41(2003)
USBio References
No references available
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