034445
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
HuAccession #
NP_004753.1; NP_059430.2Gene ID
9267Gene #
CYTH1Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-CYTH1, NT (CYTH1, D17S811E, PSCD1, Cytohesin-1, PH, SEC7 and coiled-coil domain-containing protein 1, SEC7 homolog B2-1)
CYTH1, D17S811E, PSCD1, Cytohesin-1, PH, SEC7 and coiled-coil domain-containing protein 1, SEC7 homolog B2-1
The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. This gene is highly expressed in natural killer and peripheral T cells, and regulates the adhesiveness of integrins at the plasma membrane of lymphocytes. The encoded protein is 83% homologous to that of CYTH2.
Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
CYTH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-65 amino acids from the N-terminal region of human CYTH1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
El Azreq, M.A., et al. J. Immunol. 184(2):637-649(2010)|Quast, T., et al. Blood 113(23):5801-5810(2009)|Sendide, K., et al. J. Immunol. 174(7):4210-4219(2005)|Boehm, T., et al. EMBO J. 22(5):1014-1024(2003)|Mansour, M., et al. J. Biol. Chem. 277(35):32302-32309(2002)USBio References
No references available