Technical Data

034460
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_001335.1
Gene ID
1603
Gene #
DAD1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-DAD1, CT (DAD1, Dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit DAD1, Defender against cell death 1)
DAD1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1, Defender against cell death 1

DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes. [provided by RefSeq].

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
DAD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-92 amino acids from the C-terminal region of human DAD1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)|Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)|Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :|Kulke, M.H., et al. Genes Chromosomes Cancer 47(7):591-603(2008)|Shibatani, T., et al. Biochemistry 44(16):5982-5992(2005)
USBio References
No references available
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