Technical Data

034480
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_001005375.1; NP_065153.1
Gene ID
57135
Gene #
DAZ4
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-DAZ4, ID (DAZ4, Deleted in azoospermia protein 4)
DAZ4, Deleted in azoospermia protein 4

This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains two copies of the 10.8 kb repeat. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].

Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
DAZ4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-120 amino acids from the Central region of human DAZ4.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Kim, B., et al. Hum. Reprod. 24(6):1507-1515(2009)|Lardone, M.C., et al. Fertil. Steril. 88(5):1318-1326(2007)|A, Z.C., et al. Yi Chuan 28(9):1057-1060(2006)|Kuo, P.L., et al. Fertil. Steril. 81(4):1034-1040(2004)|Ferlin, A., et al. Fertil. Steril. 81(4):1013-1018(2004)
USBio References
No references available
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