Technical Data

034504
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Gene ID
1632
Gene #
DCI
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-DCI, CT (Delta(3),Delta(2)-enoyl-CoA isomerase, D3,D2-enoyl-CoA isomerase, Dodecenoyl-CoA isomerase, 3,2-trans-enoyl-CoA isomerase, mitochondrial, DCI)
Delta(3),Delta(2)-enoyl-CoA isomerase, D3,D2-enoyl-CoA isomerase, Dodecenoyl-CoA isomerase, 3,2-trans-enoyl-CoA isomerase, mitochondrial, DCI

DCI encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates.

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
DCI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human DCI.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Partanen,S.T., et.al., J. Mol. Biol. 342 (4), 1197-1208 (2004)|Wistow,G., et.al, Mol. Vis. 8, 171-184 (2002)
USBio References
No references available
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