Technical Data

034515
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_054745.1
Gene ID
28960
Gene #
DCPS
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-DCPS, ID (DCPS, DCS1, HINT5, m7GpppX diphosphatase, DCS-1, Hint-related 7meGMP-directed hydrolase, Histidine triad protein member 5, Scavenger mRNA-decapping enzyme DcpS)
DCPS, DCS1, HINT5, m7GpppX diphosphatase, DCS-1, Hint-related 7meGMP-directed hydrolase, Histidine triad protein member 5, Scavenger mRNA-decapping enzyme DcpS

Necessary for the complete degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA fragments shorter than 10 nucleotides that are produced by 3'->5' exosome-mediated mRNA decay. Releases m7GMP. Can also degrade m7GDP to m7GMP. Has no activity towards mRNA molecules longer than 25 nucleotides.

Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 165-193 in the central region of human DCPS. Species Sequence Homology: bovine, mouse, porcine and rat.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human DCPS.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Sebastiani, P., et al. Science (2010). 2. Mariller, C., et al. Biochimie 91(1):109-122(2009). 3. Liu, S.W., et al. J. Biol. Chem. 283(24):16427-16436(2008). 4. Shen, V., et al. RNA 14(6):1132-1142(2008). 5. Lamesch, P., et al. Genomics 89(3):307-315(2007)
USBio References
No references available
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