Technical Data

034596
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E FC IHC WB
Crossreactivity
Hu
Gene ID
1677
Gene #
DFFB
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-DFFB, NT (DFFB, CAD, DFF2, DFF40, DNA fragmentation factor subunit beta, Caspase-activated deoxyribonuclease, Caspase-activated nuclease, DNA fragmentation factor 40kD subunit)
DFFB, CAD, DFF2, DFF40, DNA fragmentation factor subunit beta, Caspase-activated deoxyribonuclease, Caspase-activated nuclease, DNA fragmentation factor 40kD subunit

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.

Applications
Suitable for use in Western Blot, Immunohistochemistry, Indirect Flow Cytometry
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Indirect Flow Cytometry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
DFFB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human DFFB.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Hanus, J., et al. Apoptosis 13(3):377-382(2008)|Kalinowska-Herok, M., et al. Acta Biochim. Pol. 55(1):21-26(2008)|Neimanis, S., et al. J. Biol. Chem. 282(49):35821-35830(2007)|Hristoskova, S., et al. J. Cell. Physiol. 213(2):490-494(2007)|Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007)
USBio References
No references available
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