Technical Data

034834
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E FC WB
Crossreactivity
Hu Mo
Accession #
NP_057532.2
Gene ID
51514
Gene #
DTL
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-DTL, ID (DTL, CDT2, CDW1, DCAF2, L2DTL, RAMP, Denticleless protein homolog, DDB1- and CUL4-associated factor 2, Lethal(2) denticleless protein homolog, Retinoic acid-regulated nuclear matrix-associated protein)
DTL, CDT2, CDW1, DCAF2, L2DTL, RAMP, Denticleless protein homolog, DDB1- and CUL4-associated factor 2, Lethal(2) denticleless protein homolog, Retinoic acid-regulated nuclear matrix-associated protein

Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.

Applications
Suitable for use in Western Blot, Indirect Flow Cytometry and ELISA. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Indirect Flow Cytometry: 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 229-256 in the central region of human DTL.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human DTL. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Centore, R.C., et al. Mol. Cell 40(1):22-33(2010). 2. Abbas, T., et al. Mol. Cell 40(1):9-21(2010). 3. Song, B., et al. Mol. Cancer 9, 96 (2010). 4. Li, J., et al. Br. J. Cancer 101(4):691-698(2009). 5. Abbas, T., et al. Genes Dev. 22(18):2496-2506(2008)
USBio References
No references available
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