Technical Data

034957-FITC
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Conjugate
FITC
Isotype
IgG
Grade
Affinity Purified
Applications
FLISA WB
Crossreactivity
Hu
Accession #
NP_001954.2
Gene ID
1950
Gene #
EGF
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Rabbit Anti-EGF, CT (EGF, Pro-epidermal growth factor, Epidermal growth factor, Urogastrone) (FITC)
EGF, Pro-epidermal growth factor, Epidermal growth factor, Urogastrone

This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.

Applications
Suitable for use in Western Blot, FLISA
Recommended Dilution
FLISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
EGF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 939-967 amino acids from the C-terminal region of human EGF.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
de Diesbach, M.T., et al. Exp. Cell Res. 316(19):3239-3253(2010)|Xu, Z., et al. Biochem. Biophys. Res. Commun. 401(3):376-381(2010)|Lupien, M., et al. Genes Dev. 24(19):2219-2227(2010)|Hommel, U., et al. J. Mol. Biol. 227(1):271-282(1992)|Hernandez-Sotomayor, S.M., et al. J. Membr. Biol. 128(2):81-89(1992)
USBio References
No references available
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