Technical Data

035007
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_037366.1
Gene ID
27335
Gene #
EIF3K
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-EIF3K, CT (EIF3K, EIF3S12, Eukaryotic translation initiation factor 3 subunit K, Eukaryotic translation initiation factor 3 subunit 12, Muscle-specific gene M9 protein, PLAC-24, eIF-3 p25, eIF-3 p28)
EIF3K, EIF3S12, Eukaryotic translation initiation factor 3 subunit K, Eukaryotic translation initiation factor 3 subunit 12, Muscle-specific gene M9 protein, PLAC-24, eIF-3 p25, eIF-3 p28

The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12. eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex (Mayeur et al., 2003 [PubMed 14519125]).

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
EIF3K antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-204 amino acids from the C-terminal region of human EIF3K.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 105(47):18139-18144(2008)|Lin, Y.M., et al. J. Cell. Sci. 121 (PT 14), 2382-2393 (2008) :|Masutani, M., et al. EMBO J. 26(14):3373-3383(2007)|Damoc, E., et al. Mol. Cell Proteomics 6(7):1135-1146(2007)|Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
USBio References
No references available
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