Technical Data

035087
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E FC WB
Crossreactivity
Hu Mo
Accession #
NP_996927.1; NP_996929.1; O43768
Gene ID
2029
Gene #
ENSA
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-ENSA, ID (ENSA, Alpha-endosulfine, ARPP-19e)
ENSA, Alpha-endosulfine, ARPP-19e

ENSA belongs to a highly conserved cAMP-regulated phosphoprotein (ARPP) family. This protein was identified as an endogenous ligand for the sulfonylurea receptor, ABCC8/SUR1. ABCC8 is the regulatory subunit of the ATP-sensitive potassium (KATP) channel, which is located on the plasma membrane of pancreatic beta cells and plays a key role in the control of insulin release from pancreatic beta cells. This protein is thought to be an endogenous regulator of KATP channels. In vitro studies have demonstrated that this protein modulates insulin secretion through the interaction with KATP channel, and this gene has been proposed as a candidate gene for type 2 diabetes.

Applications
Suitable for use in Western Blot, Indirect Flow Cytometry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Indirect Flow Cytometry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
ENSA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-74 amino acids from the Central region of human ENSA.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human, mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :|Olsen, J.V., et al. Cell 127(3):635-648(2006)|Olsen, J.V., et al. Cell 127(3):635-648(2006)|Gabrielsson, B.G., et al. Mol. Cell. Biochem. 258 (1-2), 65-71 (2004) :|Thameem, F., et al. Mol. Genet. Metab. 81(1):16-21(2004)
USBio References
No references available
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