Technical Data

035094-Biotin
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Conjugate
Biotin
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_001239.2
Gene ID
956
Gene #
ENTPD3
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Rabbit Anti-ENTPD3, CT (ENTPD3, CD39L3, Ectonucleoside triphosphate diphosphohydrolase 3, CD39 antigen-like 3, Ecto-ATP diphosphohydrolase 3, Ecto-apyrase 3, HB6) (Biotin)
ENTPD3, CD39L3, Ectonucleoside triphosphate diphosphohydrolase 3, CD39 antigen-like 3, Ecto-ATP diphosphohydrolase 3, Ecto-apyrase 3, HB6

ENTPD3 is similar to E-type nucleotidases (NTPases). NTPases, such as CD39, mediate catabolism of extracellular nucleotides. ENTPD3 contains 4 apyrase-conserved regions which is characteristic of NTPases.

Applications
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other potential application, though not tested is Direct Flow Cytometry. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: Formalin-fixed, paraffin-embedded sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 496-527 in the C-terminal region of human ENTPD3.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human ENTPD3.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Fausther, M., et al. Am. J. Physiol. Lung Cell Mol. Physiol. 298 (6), L804-L818 (2010). 2. Gaddie, K.J., et al. Biochemistry 48(40):9437-9447 (2009). 3. Munkonda, M.N., et al. FEBS J. 276(2):479-496 (2009). 4. Munkonda, M.N., et al. Biochem. Pharmacol. 74(10):1524-1534 (2007). 5. Ivanenkov, V.V., et al. Biochemistry 44(25):8998-9012 (2005)
USBio References
No references available
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