035223
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E IHC WBCrossreactivity
HuAccession #
NP_001007254.2Gene ID
2086Gene #
ERV3Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-ERV3, CT (ERV3-1, ERV3, HERV-R_7q21.2 provirus ancestral Env polyprotein, ERV-3 envelope protein, ERV3-1 envelope protein, Envelope polyprotein, HERV-R envelope protein, Surface protein, Transmembrane protein)
ERV3-1, ERV3, HERV-R_7q21.2 provirus ancestral Env polyprotein, ERV-3 envelope protein, ERV3-1 envelope protein, Envelope polyprotein, HERV-R envelope protein, Surface protein, Transmembrane protein
The human genome includes many retroelements including the human endogenous retroviruses (HERVs). ERV3, one of the most studied HERVs, is thought to have integrated 30 to 40 million years ago and is present in higher primates with the exception of gorillas. Taken together, the observation of genome conservation, the detection of transcript expression, and the presence of conserved ORFs is circumstantial evidence for a functional role. A functional role is also suggested by the observation that downregulation of ERV3 is reported in choriocarcinoma. [provided by RefSeq].
Applications
Suitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 445-474 in the C-terminal region of human ERV3.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human ERV3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Andersson, A.C., et al. J. Virol. 79(14):9270-9284(2005) 2. Herve, C.A., et al. Genomics 83(5):940-943(2004) 3. Blaise, S., et al. Proc. Natl. Acad. Sci. U.S.A. 100(22):13013-13018(2003) 4. de Parseval, N., et al. J. Virol. 77(19):10414-10422(2003) 5. Andersson, A.C., et al. Virology 297(2):220-225(2002)USBio References
No references available