035529
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E IHC WBCrossreactivity
HuAccession #
NP_001019386.1; NP_060026.2Gene ID
54751Gene #
FBLIM1Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-FBLIM1, CT (FBLIM1, FBLP1, Filamin-binding LIM protein 1, Migfilin, Mitogen-inducible 2-interacting protein)
FBLIM1, FBLP1, Filamin-binding LIM protein 1, Migfilin, Mitogen-inducible 2-interacting protein
This gene encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms.
Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
FBLIM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 301-331 amino acids from the C-terminal region of human FBLIM1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Zhao, J., et al. J. Biol. Chem. 284(49):34308-34320(2009)|Ithychanda, S.S., et al. J. Biol. Chem. 284(7):4713-4722(2009)|Lad, Y., et al. J. Biol. Chem. 283(50):35154-35163(2008)|Lai-Cheong, J.E., et al. J. Invest. Dermatol. 128(9):2156-2165(2008)|Papachristou, D.J., et al. Histopathology 51(4):499-508(2007)USBio References
No references available