Technical Data

035641
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
FC IF IHC WB
Crossreactivity
Hu Mo
Gene ID
2263
Gene #
FGFR2
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-FGFR2, NT (FGFR2, BEK, KGFR, KSAM, Fibroblast growth factor receptor 2, K-sam, Keratinocyte growth factor receptor, CD332)
FGFR2, BEK, KGFR, KSAM, Fibroblast growth factor receptor 2, K-sam, Keratinocyte growth factor receptor, CD332

FGFR2 is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in the gene are associated with many craniosynostotic syndromes and bone malformations. The genomic organization of the gene encompasses 20 exons. Alternative splicing in multiple exons, including those encoding the Ig-like domains, the transmembrane region and the carboxyl terminus, results in varied isoforms which differ in structure and specificity. Isoform 1 has equal affinity for aFGF and bFGF but does not bind KGF.

Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Indirect Flow Cytometry. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1:1000-1:2000 Immunohistochemistry (FFPE): 1:50-1:100 Indirect Flow Cytometry: 1:25 Immunofluorescence: 1:25 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 22-51 in the N-terminal region of human FGFR2
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human FGFR2. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Freeman, K.W., et al., Cancer Res. 63(19):6237-6243 (2003). 2. Goriely, A., et al., Science 301(5633):643-646 (2003). 3. Fomenkov, A., et al., J. Biol. Chem. 278(26):23906-23914 (2003). 4. Katoh, M., et al., Int. J. Mol. Med. 11(5):579-583 (2003). 5. Katoh, M., et al., Int. J. Oncol. 22(5):1155-1159 (2003).
USBio References
No references available
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