Technical Data

035711-HRP
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Conjugate
HRP
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_000793.1
Gene ID
2348
Gene #
FOLR1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Rabbit Anti-FOLR1, NT (FOLR1, FOLR, Folate receptor alpha, Adult folate-binding protein, Folate receptor 1, Folate receptor, adult, KB cells FBP, Ovarian tumor-associated antigen MOv18) (Azide free) (HRP)
FOLR1, FOLR, Folate receptor alpha, Adult folate-binding protein, Folate receptor 1, Folate receptor, adult, KB cells FBP, Ovarian tumor-associated antigen MOv18

The protein encoded by this gene is a member of the folate receptor family. Members of this gene family bind folic acid and its reduced derivatives, and transport 5-methyltetrahydrofolate into cells. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. Mutations in this gene have been associated with neurodegeneration due to cerebral folate transport deficiency. Due to the presence of two promoters, multiple transcription start sites, and alternative splicing, multiple transcript variants encoding the same protein have been found for this gene.

Applications
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry (Leica): Paraffin-embedded sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.  For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 33-68 in the N-terminal region of human FOLR1.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish peroxidase (HRP).
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human FOLR1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Sivakumaran, S., et al. J. Steroid Biochem. Mol. Biol. 122(5):333-340(2010). 2. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 3. O'Byrne, M.R., et al. Birth Defects Res. Part A Clin. Mol. Teratol. 88(8):689-694(2010). 4. Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010). 5. Elwood, P.C., et al. Biochemistry 36(6):1467-1478(1997)
USBio References
No references available
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