Technical Data

035826
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_060239.2
Gene ID
55632
Gene #
G2E3
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-G2E3, ID (G2E3, KIAA1333, G2/M phase-specific E3 ubiquitin-protein ligase)
G2E3, KIAA1333, G2/M phase-specific E3 ubiquitin-protein ligase

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Essential in early embryonic development to prevent apoptotic death.

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
G2E3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-252 amino acids from the Central region of human G2E3.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Brooks, W.S., et al. J. Biol. Chem. 283(32):22304-22315(2008)|Brooks, W.S., et al. Exp. Cell Res. 313(4):665-676(2007)|Lehner, B., et al. Genomics 83(1):153-167(2004)|Crawford, D.F., et al. J. Biol. Chem. 276(40):37166-37177(2001)
USBio References
No references available
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