Technical Data

035913
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_127460.1
Gene ID
54433
Gene #
GAR1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-GAR1, ID (GAR1, NOLA1, H/ACA ribonucleoprotein complex subunit 1, Nucleolar protein family A member 1, snoRNP protein GAR1)
GAR1, NOLA1, H/ACA ribonucleoprotein complex subunit 1, Nucleolar protein family A member 1, snoRNP protein GAR1

This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA2 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. These four H/ACA snoRNP proteins are also components of the telomerase complex. The encoded protein of this gene contains two glycine- and arginine-rich domains and is related to Saccharomyces cerevisiae Gar1p. Two splice variants have been found for this gene.

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
GAR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85-114 amino acids from the Central region of human GAR1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010)|Hamma, T., et al. J. Biol. Chem. 285(2):805-809(2010)|Pigullo, S., et al. Pediatr Blood Cancer 52(3):376-378(2009)|Vasan, R.S., et al. BMC Med. Genet. 8 SUPPL 1, S2 (2007) :|Andersen, J.S., et al. Nature 433(7021):77-83(2005)
USBio References
No references available
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