Technical Data

036059
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_976325.1; NP_001121599.1
Gene ID
2710
Gene #
GK
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-GK, NT (GK, Glycerol kinase, ATP:glycerol 3-phosphotransferase)
GK, Glycerol kinase, ATP:glycerol 3-phosphotransferase

The product of this gene belongs to the FGGY kinase family of proteins and encodes glycerol kinase. Glycerol kinase is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Defects in this gene are the cause of glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been identified.

Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
GK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-51 amino acids from the N-terminal region of human GK.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Lu, Y., et al. J. Lipid Res. 49(12):2582-2589(2008)|Zhang, Y.H., et al. Pediatr. Res. 59 (4 PT 1), 590-592 (2006) :|Ohira, R.H., et al. Biochem. Biophys. Res. Commun. 331(1):239-246(2005)|Stepanian, S.V., et al. Mol. Genet. Metab. 80(4):412-418(2003)|Hellerud, C., et al. Clin. Chem. Lab. Med. 41(1):46-55(2003)
USBio References
No references available
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