Technical Data

036081
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_115964.2
Gene ID
84662
Gene #
GLIS2
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-GLIS2, ID (GLIS2, NKL, Zinc finger protein GLIS2, GLI-similar 2, Neuronal Krueppel-like protein)
GLIS2, NKL, Zinc finger protein GLIS2, GLI-similar 2, Neuronal Krueppel-like protein

This gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear transcription factor with five C2H2-type zinc finger domains. The protein encoded by this gene is widely expressed at low levels in the neural tube and peripheral nervous system and likely promotes neuronal differentiation. It is abundantly expressed in the kidney and may have a role in the regulation of kidney morphogenesis. p120 regulates the expression level of this protein and induces the cleavage of this protein's C-terminal zinc finger domain. This protein also promotes the nuclear translocation of p120. Mutations in this gene cause nephronophthisis (NPHP), an autosomal recessive kidney disease characterized by tubular basement membrane disruption, interstitial lymphohistiocytic cell infiltration, and development of cysts at the corticomedullary border of the kidneys.

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
GLIS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 172-202 amino acids from the Central region of human GLIS2.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Attanasio, M., et al. Nat. Genet. 39(8):1018-1024(2007)|Hosking, C.R., et al. Mol. Biol. Cell 18(5):1918-1927(2007)|Kim, Y.S., et al. FEBS Lett. 581(5):858-864(2007)|Olsen, J.V., et al. Cell 127(3):635-648(2006)|Kim, Y.S., et al. Nucleic Acids Res. 31(19):5513-5525(2003)
USBio References
No references available
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