Technical Data

036186-PE
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Conjugate
PE
Isotype
IgG
Grade
Affinity Purified
Applications
FLISA IHC WB
Crossreactivity
Hu
Accession #
NP_003792.1
Gene ID
8733
Gene #
GPAA1
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
Rabbit Anti-GPAA1, NT (GPAA1, GAA1, Glycosylphosphatidylinositol anchor attachment 1 protein, GAA1 protein homolog) (PE)
GPAA1, GAA1, Glycosylphosphatidylinositol anchor attachment 1 protein, GAA1 protein homolog

Posttranslational glycosylphosphatidylinositol (GPI) anchor attachment serves as a general mechanism for linking proteins to the cell surface membrane. The protein encoded by this gene presumably functions in GPI anchoring at the GPI transfer step. The mRNA transcript is ubiquitously expressed in both fetal and adult tissues. The anchor attachment protein 1 contains an N-terminal signal sequence, 1 cAMP- and cGMP-dependent protein kinase phosphorylation site, 1 leucine zipper pattern, 2 potential N-glycosylation sites, and 8 putative transmembrane domains.

Applications
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry (Not Tested) and FLISA. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: Formalin-fixed, paraffin-embedded sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 46-73 in the N-terminal region of human GPAA1, Swiss/UniProt Accession: O43292.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human GPAA1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Jiang, W.W., et al. Mol. Cancer 6, 74 (2007) : 2. Olsen, J.V., et al. Cell 127(3):635-648(2006) 3. Ho, J.C., et al. Int. J. Cancer 119(6):1330-1337(2006) 4. Vainauskas, S., et al. J. Biol. Chem. 280(16):16402-16409(2005) 5. Vainauskas, S., et al. J. Biol. Chem. 279(8):6540-6545(2004)
USBio References
No references available
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