Technical Data

036186
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E FC IHC WB
Crossreactivity
Hu
Accession #
NP_003792.1
Gene ID
8733
Gene #
GPAA1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-GPAA1, NT (GPAA1, GAA1, Glycosylphosphatidylinositol anchor attachment 1 protein, GAA1 protein homolog)
GPAA1, GAA1, Glycosylphosphatidylinositol anchor attachment 1 protein, GAA1 protein homolog

Posttranslational glycosylphosphatidylinositol (GPI) anchor attachment serves as a general mechanism for linking proteins to the cell surface membrane. The protein encoded by this gene presumably functions in GPI anchoring at the GPI transfer step. The mRNA transcript is ubiquitously expressed in both fetal and adult tissues. The anchor attachment protein 1 contains an N-terminal signal sequence, 1 cAMP- and cGMP-dependent protein kinase phosphorylation site, 1 leucine zipper pattern, 2 potential N-glycosylation sites, and 8 putative transmembrane domains.

Applications
Suitable for use in Western Blot, Immunohistochemistry, Indirect Flow Cytometry and ELISA. Other applications have not been tested.
Recommended Dilution
Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:50-1:100 Indirect Flow Cytometry: 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 46-73 in the N-terminal region of human GPAA1, Swiss/UniProt Accession: O43292.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human GPAA1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Jiang, W.W., et al. Mol. Cancer 6, 74 (2007) : 2. Olsen, J.V., et al. Cell 127(3):635-648(2006) 3. Ho, J.C., et al. Int. J. Cancer 119(6):1330-1337(2006) 4. Vainauskas, S., et al. J. Biol. Chem. 280(16):16402-16409(2005) 5. Vainauskas, S., et al. J. Biol. Chem. 279(8):6540-6545(2004)
USBio References
No references available
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