Technical Data

036269-FITC
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Conjugate
FITC
Isotype
IgG
Grade
Affinity Purified
Applications
FLISA WB
Crossreactivity
Hu Mo
Accession #
NP_001135938.1
Gene ID
84706
Gene #
GPT2
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Rabbit Anti-GPT2, NT (GPT2, AAT2, ALT2, Alanine aminotransferase 2, Glutamate pyruvate transaminase 2, Glutamic-alanine transaminase 2, Glutamic-pyruvic transaminase 2) (FITC)
GPT2, AAT2, ALT2, Alanine aminotransferase 2, Glutamate pyruvate transaminase 2, Glutamic--alanine transaminase 2, Glutamic--pyruvic transaminase 2

GPT (MIM 138200) and GPT2 (EC 2.6.1.2), also known as alanine transaminases, are pyridoxal enzymes that catalyze the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. By mediating the conversion of these 4 major intermediate metabolites, these transaminases have roles in gluconeogenesis and in amino acid metabolism.

Applications
Suitable for use in Western Blot and FLISA. Other applications have not been tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 15-44 in the N-terminal region of human GPT2.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human GPT2. Species Crossreactivity: mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Glinghammar, B., et al. Int. J. Mol. Med. 23(5):621-631(2009) 2. Fraser, A., et al. Hepatology 46(1):158-165(2007) 3. Ding, Y., et al. World J. Gastroenterol. 12(43):7038-7041(2006) 4. Martin, J., et al. Nature 432(7020):988-994(2004) 5. Yang, R.Z., et al. Genomics 79(3):445-450(2002)
USBio References
No references available
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