036285
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
PurifiedApplications
E IHC WBCrossreactivity
Hu MoAccession #
O70326Gene ID
23892Gene #
GREM1; Grem1Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-GREMLIN, CT (Grem1, Cktsf1b1, Drm, Gremlin-1, Cysteine knot superfamily 1, BMP antagonist 1, Down-regulated in Mos-transformed cells protein)
Grem1, Cktsf1b1, Drm, Gremlin-1, Cysteine knot superfamily 1, BMP antagonist 1, Down-regulated in Mos-transformed cells protein
GREMLIN is a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to relay the sonic hedgehog (SHH) signal from the polarizing region to the apical ectodermal ridge during limb bud outgrowth.
Applications
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested.
Recommended Dilution
Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 147-175 in the C-terminal region of human Gremlin, UniProt #O70326. Species Sequence Homology: rat
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Ammonium Sulfate Precipitation.
Specificity
Recognizes human Gremlin. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Chen, B., et al., J. Immunol. 173(10):5914-5917 (2004). 2. Chen, B., et al., Biochem. Biophys. Res. Commun. 295(5):1135-1141 (2002).3. McMahon, R., et al., J. Biol. Chem. 275(14):9901-9904 (2000). 4. Murphy, M., et al., J. Biol. Chem. 274(9):5830-5834 (1999). 5. Hsu, D.R., et al., Mol. Cell 1(5):673-683 (1998).USBio References
No references available