Technical Data

036332
Clone Type
Polyclonal
Host
Rabbit
Source
Mouse
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Mo
Accession #
NP_062801.1
Gene ID
56637
Gene #
Gsk3b
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Gsk3b, NT (Gsk3b, Glycogen synthase kinase-3 beta, Serine/threonine-protein kinase GSK3B)
Gsk3b, Glycogen synthase kinase-3 beta, Serine/threonine-protein kinase GSK3B

Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. May phosphorylate MUC1 and decrease the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1 (By similarity).

Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Mouse Gsk3b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-59 amino acids from the N-terminal region of Mouse Gsk3b.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Liu, Y., et al. Diabetologia 53(12):2600-2610(2010)|Graham, J.A., et al. J. Biol. Chem. 285(43):32852-32859(2010)|Ahmad, I., et al. Oncogene (2010) In press :|Asada, N., et al. J. Neurosci. 30(26):8852-8865(2010)|Carew, R.M., et al. BMC Dev. Biol. 10, 73 (2010) :
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net