Technical Data

036354
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_005504.2
Gene ID
2960
Gene #
GTF2E1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-GTF2E1, NT (GTF2E1, TF2E1, General transcription factor IIE subunit 1, General transcription factor IIE 56kD subunit, Transcription initiation factor IIE subunit alpha)
GTF2E1, TF2E1, General transcription factor IIE subunit 1, General transcription factor IIE 56kD subunit, Transcription initiation factor IIE subunit alpha

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.

Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
GTF2E1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-121 amino acids from the N-terminal region of human GTF2E1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Vega, A., et al. Gynecol. Oncol. 112(1):210-214(2009)|Okuda, M., et al. EMBO J. 27(7):1161-1171(2008)|Di Lello, P., et al. Proc. Natl. Acad. Sci. U.S.A. 105(1):106-111(2008)|Jawhari, A., et al. EMBO Rep. 7(5):500-505(2006)|Okuda, M., et al. J. Biol. Chem. 279(49):51395-51403(2004)
USBio References
No references available
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